Single Molecules Observed by Near-Field Scanning Optical Microscopy

@article{Betzig1993SingleMO,
  title={Single Molecules Observed by Near-Field Scanning Optical Microscopy},
  author={E. Betzig and R. J. Chichester},
  journal={Science},
  year={1993},
  volume={262},
  pages={1422 - 1425}
}
Individual carbocyanine dye molecules in a sub-monolayer spread have been imaged with near-field scanning optical microscopy. Molecules can be repeatedly detected and spatially localized (to ∼λ/50 where λ is the wavelength of light) with a sensitivity of at least 0.005 molecules/(Hz)1/2 and the orientation of each molecular dipole can be determined. This information is exploited to map the electric field distribution in the near-field aperture with molecular spatial resolution. 
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Topics from this paper

Near-field optical microscopy for subwavelength investigation of biological structures
Field enhancement and apertureless near‐field optical spectroscopy of single molecules
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