Single-Molecule mRNA Decay Measurements Reveal Promoter- Regulated mRNA Stability in Yeast

@article{Trcek2011SingleMoleculeMD,
  title={Single-Molecule mRNA Decay Measurements Reveal Promoter- Regulated mRNA Stability in Yeast},
  author={Tatjana Trcek and Daniel R Larson and Alberto Mold{\'o}n and Charles C. Query and Robert H Singer},
  journal={Cell},
  year={2011},
  volume={147},
  pages={1484-1497}
}
Messenger RNA decay measurements are typically performed on a population of cells. However, this approach cannot reveal sufficient complexity to provide information on mechanisms that may regulate mRNA degradation, possibly on short timescales. To address this deficiency, we measured cell cycle-regulated decay in single yeast cells using single-molecule FISH. We found that two genes responsible for mitotic progression, SWI5 and CLB2, exhibit a mitosis-dependent mRNA stability switch. Their… CONTINUE READING