Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time.

@article{Kong2017SingleMoleculeMF,
  title={Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time.},
  author={Muwen Kong and Emily C Beckwitt and Luke Springall and Neil M. Kad and Bennett Van Houten},
  journal={Methods in enzymology},
  year={2017},
  volume={592},
  pages={213-257}
}
Single-molecule approaches to solving biophysical problems are powerful tools that allow static and dynamic real-time observations of specific molecular interactions of interest in the absence of ensemble-averaging effects. Here, we provide detailed protocols for building an experimental system that employs atomic force microscopy and a single-molecule DNA tightrope assay based on oblique angle illumination fluorescence microscopy. Together with approaches for engineering site-specific lesions… CONTINUE READING
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