Single Molecule Cluster Analysis dissects splicing pathway conformational dynamics

  title={Single Molecule Cluster Analysis dissects splicing pathway conformational dynamics},
  author={Mario R. Blanco and Joshua S. Martin and Matthew L. Kahlscheuer and Ramya Krishnan and John Abelson and Alain Laederach and Nils G Walter},
  journal={Nature Methods},
We report Single Molecule Cluster Analysis (SiMCAn), which utilizes hierarchical clustering of hidden Markov modeling–fitted single-molecule fluorescence resonance energy transfer (smFRET) trajectories to dissect the complex conformational dynamics of biomolecular machines. We used this method to study the conformational dynamics of a precursor mRNA during the splicing cycle as carried out by the spliceosome. By clustering common dynamic behaviors derived from selectively blocked splicing… CONTINUE READING
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