Single Fish Egg DNA Extraction for PCR Amplification

  title={Single Fish Egg DNA Extraction for PCR Amplification},
  author={Futoshi Aranishi},
  journal={Conservation Genetics},
Modern stock researches on marine biomass are basically genetic and rely increasingly on PCR-based manipulations of informative DNA markers for detecting the genetic diversity. This study developed a simple and rapid single tube method for DNA extraction from a single fish egg. The 15 min protocol was based on the use of Chelex 100 resin and urea to breakdown membrane and connective tissue of eggs. From various sizes of a single egg of walleye pollack (Theragra chalcogramma), the amounts of… CONTINUE READING
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Phylogenetic analysis of the subclass Pteriomorphia (Bivalvia) from mtDNA COI sequences

  • M Matsumoto
  • Molecular Phylogenetics and Evolution,
  • 2003
2 Excerpts

Screening of DNA polymorphisms in samples of archived scales from New Zealand snapper

  • GJ Adcock, Ramirez JHB, L Hauser, P Smith, GR Carvalho
  • Journal of Fish Biology
  • 2000
1 Excerpt

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