Single Fish Egg DNA Extraction for PCR Amplification

@article{Aranishi2005SingleFE,
  title={Single Fish Egg DNA Extraction for PCR Amplification},
  author={Futoshi Aranishi},
  journal={Conservation Genetics},
  year={2005},
  volume={7},
  pages={153-156}
}
Modern stock researches on marine biomass are basically genetic and rely increasingly on PCR-based manipulations of informative DNA markers for detecting the genetic diversity. This study developed a simple and rapid single tube method for DNA extraction from a single fish egg. The 15 min protocol was based on the use of Chelex 100 resin and urea to breakdown membrane and connective tissue of eggs. From various sizes of a single egg of walleye pollack (Theragra chalcogramma), the amounts of… CONTINUE READING
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