Single-Cell Whole-Genome Amplification and Sequencing: Methodology and Applications.

  title={Single-Cell Whole-Genome Amplification and Sequencing: Methodology and Applications.},
  author={L. Huang and F. Ma and Alec R. Chapman and S. Lu and X. Xie},
  journal={Annual review of genomics and human genetics},
  • L. Huang, F. Ma, +2 authors X. Xie
  • Published 2015
  • Biology, Medicine
  • Annual review of genomics and human genetics
  • We present a survey of single-cell whole-genome amplification (WGA) methods, including degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), multiple displacement amplification (MDA), and multiple annealing and looping-based amplification cycles (MALBAC). The key parameters to characterize the performance of these methods are defined, including genome coverage, uniformity, reproducibility, unmappable rates, chimera rates, allele dropout rates, false positive rates for calling… CONTINUE READING
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