Single-Cell Real-Time Visualization and Quantification of Perylene Bioaccumulation in Microorganisms.

@article{Jin2017SingleCellRV,
  title={Single-Cell Real-Time Visualization and Quantification of Perylene Bioaccumulation in Microorganisms.},
  author={Xin Jin and Xuejun Guo and Deshu Xu and Yanna Zhao and Xinghui Xia and Fengshan Bai},
  journal={Environmental science & technology},
  year={2017},
  volume={51 11},
  pages={
          6211-6219
        }
}
Bioaccumulation of perylene in Escherichia coli and Staphylococcus aureus was visualized and quantified in real time with high sensitivity at high temporal resolution. For the first time, single-molecule fluorescence microscopy (SMFM) with a microfluidic flow chamber and temperature control has enabled us to record the dynamic process of perylene bioaccumulation in single bacterial cells and examine the cell-to-cell heterogeneity. Although with identical genomes, individual E. coli cells… CONTINUE READING
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