Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions


We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection… (More)
DOI: 10.1371/journal.pone.0002061


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