Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.

@article{Neri2015SingleBaseRA,
  title={Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.},
  author={Francesco Neri and Danny Incarnato and Anna Křepelov{\'a} and Stefania Rapelli and Francesca Anselmi and Caterina Parlato and Claudio Medana and Federica Dal Bello and Salvatore Oliviero},
  journal={Cell reports},
  year={2015}
}
Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg). Here, we present a genome-wide approach, named methylation-assisted bisulfite sequencing (MAB-seq), that enables single-base resolution mapping of 5fC and 5caC and measures their abundance. Application of this method to mouse embryonic stem cells (ESCs) shows the… CONTINUE READING
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