Single‐channel analysis of an NMDA receptor possessing a mutation in the region of the glutamate binding site

  title={Single‐channel analysis of an NMDA receptor possessing a mutation in the region of the glutamate binding site},
  author={L. Anson and R. Schoepfer and D. Colquhoun and D. Wyllie},
  journal={The Journal of Physiology},
  • L. Anson, R. Schoepfer, +1 author D. Wyllie
  • Published 2000
  • Chemistry, Medicine
  • The Journal of Physiology
  • 1 Recombinant NR1a/NR2A(T671A) N‐methyl‐D‐aspartate (NMDA) receptor‐channels, which carry a point mutation in the putative glutamate binding site that reduces glutamate potency by around 1000‐fold, have been expressed in Xenopus laevis oocytes and their single‐channel properties examined using patch‐clamp recording techniques. 2 Shut time distributions of channel activity were fitted with a mixture of five exponential components. The first three components in each distribution were considered… CONTINUE READING
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