Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis

@article{Hu2003SimultaneousVO,
  title={Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis},
  author={C H Hu and Tom Klaus Kerppola},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={539-545}
}
The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein interactions in the same cell. This approach is based on complementation between fragments of fluorescent proteins with different spectral characteristics. We have identified 12… CONTINUE READING
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