Simultaneous topographic and fluorescence imagings of recombinant bacterial cells containing a green fluorescent protein gene detected by a scanning near-field optical/atomic force microscope.

@article{Tamiya1997SimultaneousTA,
  title={Simultaneous topographic and fluorescence imagings of recombinant bacterial cells containing a green fluorescent protein gene detected by a scanning near-field optical/atomic force microscope.},
  author={Eiichi Tamiya and Shin-ichiro Iwabuchi and Naoki Nagatani and Yoichi Murakami and Tomomi Sakaguchi and Kenji Yokoyama and N. Chiba and Hiroshi Muramatsu},
  journal={Analytical chemistry},
  year={1997},
  volume={69 18},
  pages={3697-701}
}
A scanning near-field optical/atomic force microscope (SNOAM) system was applied for simultaneous topographic and fluorescence imaging of biological samples in air and liquid. The SNOAM uses a bent optical fiber simultaneously as a dynamic mode atomic force microscopy cantilever and as a scanning near-field optical microscopy probe. Optical resolution of this system was about 50-100 nm in fluorescence mode for fluorescent latex beads on a quartz glass plate. Green fluorescent protein (GFP) is a… CONTINUE READING