Simultaneous time- and wavelength-resolved fluorescence microscopy of single molecules.

@article{Luong2005SimultaneousTA,
  title={Simultaneous time- and wavelength-resolved fluorescence microscopy of single molecules.},
  author={A. Khai Luong and Claudiu C. Gradinaru and David W Chandler and Carl C. Hayden},
  journal={The journal of physical chemistry. B},
  year={2005},
  volume={109 33},
  pages={15691-8}
}
Single fluorophores and single-pair fluorescence resonance energy transfer were studied with a new confocal fluorescence microscope that allows, for the first time, the wavelength and emission time of each detected photon to be simultaneously measured with single molecule sensitivity. In this apparatus, the photons collected from the sample are imaged through a dispersive optical system onto a time and position sensitive photon detector. For each detected photon the detection system records its… CONTINUE READING