Simultaneous recording of intramembrane charge movement components and calcium release in wild-type and S100A1-/- muscle fibres.

@article{Prosser2009SimultaneousRO,
  title={Simultaneous recording of intramembrane charge movement components and calcium release in wild-type and S100A1-/- muscle fibres.},
  author={Benjamin L Prosser and Erick O Hern{\'a}ndez-Ochoa and Danna B. Zimmer and Martin F. Schneider},
  journal={The Journal of physiology},
  year={2009},
  volume={587 Pt 18},
  pages={4543-59}
}
In the preceding paper, we reported that flexor digitorum brevis (FDB) muscle fibres from S100A1 knock-out (KO) mice exhibit a selective suppression of the delayed, steeply voltage-dependent component of intra-membrane charge movement current termed Q(gamma). Here, we use 50 microm of the Ca(2+) indicator fluo-4 in the whole cell patch clamp pipette, in addition to 20 mM EGTA and other constituents included for the charge movement studies, and calculate the SR Ca(2+) release flux from the fluo… CONTINUE READING

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