Simultaneous radioimmunoassay of progesterone, androst-4-enedione, pregnenolone, dehydroepiandrosterone and 17-hydroxyprogesterone in specific regions of human brain.

@article{Lacroix1987SimultaneousRO,
  title={Simultaneous radioimmunoassay of progesterone, androst-4-enedione, pregnenolone, dehydroepiandrosterone and 17-hydroxyprogesterone in specific regions of human brain.},
  author={Claire Lacroix and Jean Fiet and Jean Pierre Benais and B Gueux and R Bon{\`e}te and Jean Marie Villette and Bernard Gourmel and Cl Dreux},
  journal={Journal of steroid biochemistry},
  year={1987},
  volume={28 3},
  pages={317-25}
}
Simultaneous determination of progesterone, androst-4-enedione, pregnenolone, dehydroepiandrosterone (DHEA) and 17-hydroxyprogesterone has been developed for human cerebral tissue. Before immunoassay, steroids were separated on a Celite column with propylene glycol as stationary phase with hexane containing increasing proportions of dichloromethane as mobile phase. This system allowed separation of steroids of similar polarity, especially of pregnenolone and progesterone. The brain regions… CONTINUE READING