Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay.

@article{Carson1999SimultaneousQO,
  title={Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay.},
  author={Richard T. Carson and Dario A. A. Vignali},
  journal={Journal of immunological methods},
  year={1999},
  volume={227 1-2},
  pages={41-52}
}
Several methods have been developed to quantify cytokines and chemokines in biological fluids and tissue culture samples, including bioassays, enzyme-linked immunosorbent assay (ELISA), intracellular staining, ribonuclease protection assay (RPA) and polymerase chain reaction (PCR). However, each of these techniques possesses one or more significant limitations. Here, we describe a new multiplexed assay, using the FlowMetrix system, that can quantify multiple cytokines simultaneously in a small… CONTINUE READING

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