Simultaneous quantification of voriconazole and posaconazole in human plasma by high-performance liquid chromatography with ultra-violet detection.

@article{Chhun2007SimultaneousQO,
  title={Simultaneous quantification of voriconazole and posaconazole in human plasma by high-performance liquid chromatography with ultra-violet detection.},
  author={St{\'e}phanie Chhun and Elisabeth Rey and Agnes Tran and Olivier Lortholary and G{\'e}rard Pons and Vincent Jullien},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2007},
  volume={852 1-2},
  pages={223-8}
}
A sensitive and selective high-performance liquid chromatographic (HPLC) method with ultra-violet detection has been developed and validated for the simultaneous determination of posaconazole and voriconazole, two systemic anti-fungal agents. An internal standard diazepam was added to 100 microL of human plasma followed by 3 mL of hexane-methylene chloride (70:30, v/v). The organic layer was evaporated to dryness and the residue was reconstituted with 100 microL of mobile phase before being… CONTINUE READING