Simultaneous quantification of two plant viruses by double-label time-resolved immunofluorometric assay.

Abstract

For simultaneous and sensitive detection of two antigens in one sample, monoclonal antibody (MAb) to potato virus M (PVM) was labelled with a lanthanide Eu3+ and MAb to potato virus X (PVX) with another lanthanide Sm3+. A mixture of the labelled MAbs was used as a tracer. After performing the immunoreactions, the fluorescence of the dissociated lanthanides… (More)

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@article{Saarma1989SimultaneousQO, title={Simultaneous quantification of two plant viruses by double-label time-resolved immunofluorometric assay.}, author={Merilin Saarma and Lilian J{\"a}rvek{\"u}lg and Ilkka A. Hemmil{\"a} and Harri Siitari and R Sinij{\"a}rv}, journal={Journal of virological methods}, year={1989}, volume={23 1}, pages={47-54} }