Simultaneous quantification of lenalidomide, ibrutinib and its active metabolite PCI-45227 in rat plasma by LC-MS/MS: application to a pharmacokinetic study.

@article{Veeraraghavan2015SimultaneousQO,
  title={Simultaneous quantification of lenalidomide, ibrutinib and its active metabolite PCI-45227 in rat plasma by LC-MS/MS: application to a pharmacokinetic study.},
  author={Sridhar Veeraraghavan and Srikant Viswanadha and Satheeshmanikandan R. S. Thappali and Babu Govindarajulu and Swaroopkumar Vakkalanka and Manivannan Rangasamy},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2015},
  volume={107},
  pages={151-8}
}
Efficacy assessments using a combination of ibrutinib and lenalidomide necessitate the development of an analytical method for determination of both drugs in plasma with precision. A high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of lenalidomide, ibrutinib, and its active metabolite PCI45227 in rat plasma. Extraction of lenalidomide, ibrutinib, PCI45227 and tolbutamide (internal standard; IS) from 50 μl rat… CONTINUE READING