Simultaneous quantification of actin monomer and filament dynamics with modeling-assisted analysis of photoactivation

@inproceedings{Kapustina2016SimultaneousQO,
  title={Simultaneous quantification of actin monomer and filament dynamics with modeling-assisted analysis of photoactivation},
  author={Maryna Kapustina and Thomas Read and Eric A Vitriol},
  booktitle={Journal of cell science},
  year={2016}
}
Photoactivation allows one to pulse-label molecules and obtain quantitative data about their behavior. We have devised a new modeling-based analysis for photoactivatable actin experiments that simultaneously measures properties of monomeric and filamentous actin in a three-dimensional cellular environment. We use this method to determine differences in the dynamic behavior of β- and γ-actin isoforms, showing that both inhabit filaments that depolymerize at equal rates but that β-actin exists in… CONTINUE READING
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