Simultaneous protein expression and modification: an efficient approach for production of unphosphorylated and biotinylated receptor tyrosine kinases by triple infection in the baculovirus expression system.

@article{Erdmann2010SimultaneousPE,
  title={Simultaneous protein expression and modification: an efficient approach for production of unphosphorylated and biotinylated receptor tyrosine kinases by triple infection in the baculovirus expression system.},
  author={Dirk H. Erdmann and Catherine C Zimmermann and Patrizia Fontana and Jean-Christophe Hau and Alain de Pover and Patrick Ch{\`e}ne},
  journal={Journal of biomolecular techniques : JBT},
  year={2010},
  volume={21 1},
  pages={9-17}
}
Protein kinases can adopt multiple protein conformations depending on their activation status. Recently, in drug discovery, a paradigm shift has been initiated, moving from inhibition of fully activated, phosphorylated kinases to targeting the inactive, unphosphorylated forms. For identification and characterization of putative inhibitors, also interacting… CONTINUE READING