Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.

@article{Akiyama2004SimultaneousMO,
  title={Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.},
  author={Tsuyoshi Akiyama and Toji Yamazaki and Hidezo Mori and Kenji Sunagawa},
  journal={Neurochemistry international},
  year={2004},
  volume={44 7},
  pages={497-503}
}
To simultaneously monitor acetylcholine release from pre-ganglionic adrenal sympathetic nerve endings and catecholamine release from post-ganglionic adrenal chromaffin cells in the in vivo state, we applied microdialysis technique to anesthetized rats. Dialysis probe was implanted in the left adrenal medulla and perfused with Ringer's solution containing neostigmine (a cholinesterase inhibitor). After transection of splanchnic nerves, we electrically stimulated splanchnic nerves or locally… CONTINUE READING
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