Simultaneous localization of LHRH and catecholamines in rat hypothalamus.

@article{Ajika1979SimultaneousLO,
  title={Simultaneous localization of LHRH and catecholamines in rat hypothalamus.},
  author={K Ajika},
  journal={Journal of anatomy},
  year={1979},
  volume={128 Pt 2},
  pages={331-47}
}
  • K Ajika
  • Published 1979 in Journal of anatomy
The PAP unlabelled antibody enzyme method of Sternberger was used for the histochemical demonstration of LHRH and the catecholamine synthesizing enzyme, tyrosine hydroxylase (TH) in the hypothalamus of the adult male and pregnant female rat. The sections for light and electron microscopy were serially treated with normal goat serum, LHRH antiserum and/or TH antiserum, goat anti-rabbit IgG, PAP complex and 3,3'-diaminobenzidine (DAB) or 4-Cl-1-naphtol. LHRH-positive cell bodies were discernible… CONTINUE READING

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