Simultaneous isolation of seven compounds from Glehnia littoralis roots by off-line overpressured layer chromatography guided by a TLC antioxidant autographic assay.

A combinative method using overpressured layer chromatography (OPLC) and TLC bioautography against O2(•-) was developed to separate antioxidants from Glehnia littoralis roots. Through target-directed isolation by the TLC bioautographic method, seven compounds including five antioxidants were rapidly isolated by OPLC and identified as 1-linoloyl-3… (More)