Simultaneous introduction of multiple mutations using overlap extension PCR.

@article{Ge1997SimultaneousIO,
  title={Simultaneous introduction of multiple mutations using overlap extension PCR.},
  author={Lynn Ge and Pierre Rudolph},
  journal={BioTechniques},
  year={1997},
  volume={22 1},
  pages={
          28-30
        }
}
Introduction of multiple mutations can be accomplished through phage M13-based site-directed mutagenesis using several oligonucleotides (6). The major drawback of this method is the low efficiency of generating all the intended mutations simultaneously (7,9). The alternative methods for introducing multisite mutations are based on polymerase chain reaction (PCR) (1) and ligase chain reaction (LCR) (8), but they require complicated modifications to the current mutagenesis and PCR protocols… CONTINUE READING

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