Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples

@article{Simons2011SimultaneousIV,
  title={Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples},
  author={Peter M. Simons and S. Michelle Young and Mark B. Carter and Anna Waller and Dayong Zhai and John C. Reed and Bruce S. Edwards and Larry A. Sklar},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={943-952}
}
The B-cell lymphoma-2 (Bcl-2) family contains six antiapoptotic members, each with a hydrophobic pocket in which Bcl-2 homology region 3 (BH3) helices bind. This binding quenches apoptotic signals from activated BH3 family members. Many tumor cells either have increased expression of one of these six proteins or become overexpressed under treatment. Six fusion proteins made up of glutathione-S-transferase and each of the Bcl-2 members are bound individually to six glutathione bead sets, each… CONTINUE READING

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