Simultaneous immobilization of glucose oxidase and peroxidase to urea derivative of regenerated acetylcellulose granules

@article{Yotova1997SimultaneousIO,
  title={Simultaneous immobilization of glucose oxidase and peroxidase to urea derivative of regenerated acetylcellulose granules},
  author={Lyubov Yotova and Ivan Petkov Ivanov},
  journal={Applied Biochemistry and Biotechnology},
  year={1997},
  volume={61},
  pages={277-287}
}
A method for simultaneous covalent immobilization of glucose oxidase and peroxidase with previously oxidized carbohydrate residues to urea derivative of regenerated acetylcellulose granules is described. The effect of immobilization on the catalytic properties of the separately immobilized enzymes are studied. The immobilized enzymes manifested no change in their pH and temperature optima and slight increase ofKmx compared to data for the soluble enzymes. A column packed with simultaneously… CONTINUE READING