Simultaneous imaging of multiple focal planes using a two-photon scanning microscope.

@article{Amir2007SimultaneousIO,
  title={Simultaneous imaging of multiple focal planes using a two-photon scanning microscope.},
  author={Wafa Amir and Ram{\'o}n Carriles and Erich E. Hoover and Thomas A. Planchon and Charles G. Durfee and Jeff A. Squier},
  journal={Optics letters},
  year={2007},
  volume={32 12},
  pages={1731-3}
}
Despite all the advances in nonlinear microscopy, all existing instruments are constrained to obtain images of one focal plane at a time. In this Letter we demonstrate a two-photon absorption fluorescence scanning microscope capable of imaging two focal planes simultaneously. This is accomplished by temporally demultiplexing the signal coming from two focal volumes at different sample depths. The scheme can be extended to three or more focal planes.