Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP

@article{Fanello2002SimultaneousIO,
  title={Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP},
  author={Caterina Fanello and Federica Santolamazza and Alessandra della Torre},
  journal={Medical and Veterinary Entomology},
  year={2002},
  volume={16}
}
Abstract For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR‐RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large‐scale processing of field‐collected larval and adult specimens to be identified in malaria vector studies. 

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The results provide a basis for future work, which is needed to accurately describe the distribution of the An.
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References

SHOWING 1-10 OF 15 REFERENCES

Identification by rDNA‐PCR of Anopheles bwambae, a geothermal spring species of the An. gambiae complex

The identification of the malaria vector Anopheles bwambaeby rDNA‐PCR is described. PCR primers that amplify a region of the intergenic spacer of rDN A of An. gambiae s.s. produce two diagnostic PCR

Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction.

A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of

Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation

TLDR
Three chromosomal forms of Anopheles gambiae s.s., designated as Bamako, Mopti and Savanna, were studied for diagnostic PCR assays based on the analysis of the X‐linked ribosomal DNA and the results confirm previous observations indicating barriers to gene flow within An.

Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa

TLDR
Chromosome‐2 inversions seem to be involved in ecotypic adaptation rather than in mate‐recognition systems in Anopheles gambiae s.s. samples collected in several African countries.

PCR assay for identification of Anopheles quadriannulatus species B from Ethiopia and other sibling species of the Anopheles gambiae complex

TLDR
This modified PCR assay can be used to distinguish between species A and B of An.

A Polytene Chromosome Analysis of the Anopheles gambiae Species Complex

TLDR
Field-collected specimens of all known taxa in the Anopheles gambiae complex were analyzed on the basis of chromosome inversions with reference to a standard polytene chromosome map, providing evidence for genetically distinct populations in A. gambiae, A. arabiensis, and A. melas.

Attempts to molecularly distinguish cryptic taxa in Anopheles gambiae s.s.

Analyses of inversions in polytene chromosomes indicate that, in West Africa, Anopheles gambiae (sensu stricto) may be a complex of more than a single taxonomic unit, and these units have been called

Speciation Within Anopheles gambiae-- the Glass Is Half Full

Restrictions to gene flow among molecular forms of the mosquito Anopheles gambiae sensu stricto reveal an ongoing speciation process affecting the epidemiology of malaria in sub-Saharan Africa.

Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae

TLDR
Polytene chromosome studies on the Afrotropical mosquitoes of the Anopheles gambiae complex show that the rearrangements of the banding pattern are all based on paracentric inversions, probably one of the most primitive in the complex.