Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP

  title={Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP},
  author={Caterina Fanello and Federica Santolamazza and Alessandra della Torre},
  journal={Medical and Veterinary Entomology},
Abstract For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR‐RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large‐scale processing of field‐collected larval and adult specimens to be identified in malaria vector studies. 

The Seasonality and Ecology of the Anopheles gambiae complex (Dipetra: Culicidae) in Liberia Using Molecular Identification

Members of the Anopheles gambiae sensu lato (Giles) complex define a group of seven morphologically indistinguishable species, including the principal malaria vectors in Sub-Saharan Africa, which is critical to monitoring and evaluating malaria threat levels.

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Findings suggest the first documented evidence of hybrid forms of An.

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Abstract Anopheles gambiae sensu lato complex (An. gambiae s.l.) describes a group of nine morphologically indistinguishable members that vary in their distribution, ability to transmit malaria, and

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Two new species within the Anopheles gambiae complex are here described and named. Based on molecular and bionomical evidence, the An. gambiae molecular "M form" is named Anopheles coluzzii Coetzee &

Presence of species within the Anopheles gambiae complex in the Democratic Republic of Congo.

The results provide a basis for future work, which is needed to accurately describe the distribution of the An.



Identification by rDNA‐PCR of Anopheles bwambae, a geothermal spring species of the An. gambiae complex

The identification of the malaria vector Anopheles bwambaeby rDNA‐PCR is described. PCR primers that amplify a region of the intergenic spacer of rDN A of An. gambiae s.s. produce two diagnostic PCR

Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction.

A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of

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Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa

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This modified PCR assay can be used to distinguish between species A and B of An.

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Analyses of inversions in polytene chromosomes indicate that, in West Africa, Anopheles gambiae (sensu stricto) may be a complex of more than a single taxonomic unit, and these units have been called

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Restrictions to gene flow among molecular forms of the mosquito Anopheles gambiae sensu stricto reveal an ongoing speciation process affecting the epidemiology of malaria in sub-Saharan Africa.

Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae

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