Simultaneous high-performance liquid chromatographic determination of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicines.

@article{Okamura1999SimultaneousHL,
  title={Simultaneous high-performance liquid chromatographic determination of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicines.},
  author={Nobuyuki Okamura and Hiromitsu Miki and Hidefumi Orii and Yoshie Masaoka and Satoru Yamashita and Hisato Kobayashi and Akira Yagi},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={1999},
  volume={19 3-4},
  pages={603-12}
}
We report a high-performance liquid chromatographic method to determine the quantities of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicine. All seven compounds were separated in less than 30 min with a Wakosil-II 5C18 AR column by linear gradient elution using 0.01% (v/v) phosphoric acid acetonitrile (0 min 90:10, 10 min 88:12, 22 min 70:30, 30 min 30:70) as the mobile phase at a flow-rate of 1.0 ml/min(-1), and detection at 250 nm… CONTINUE READING