Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems.

@article{Li2013SimultaneousGA,
  title={Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems.},
  author={Wei Li and Fei Teng and Tianda Li and Qi Zhou},
  journal={Nature biotechnology},
  year={2013},
  volume={31 8},
  pages={684-6}
}
volume 31 NumBeR 8 AuGuST 2013 nature biotechnology small RNA that can be simply expressed or transfected with the Cas9 nuclease. We chose the Tet family genes as targets to demonstrate the feasibility of CRISPRCas–mediated mutagenesis in rat. The three Tet genes encode proteins with DNA hydroxymethylase functions and play key roles in the regulation of many important biological processes11. The Cas9 endonuclease functions either with a combination of a short crRNA and a transactivating crRNA… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 172 extracted citations

Similar Papers

Loading similar papers…