Simultaneous fluorescence in situ hybridization of mRNA and rRNA for the detection of gene expression in environmental microbes.

@article{Pernthaler2005SimultaneousFI,
  title={Simultaneous fluorescence in situ hybridization of mRNA and rRNA for the detection of gene expression in environmental microbes.},
  author={Annelie Pernthaler and Jakob Pernthaler},
  journal={Methods in enzymology},
  year={2005},
  volume={397},
  pages={352-71}
}
A protocol is presented for the detection of gene expression in environmental microorganisms by means of fluorescence in situ hybridization (FISH). Messenger RNA (mRNA) is hybridized with digoxigenin (DIG)- or fluorescein (FLUOS)-labeled ribonucleotide probes. Subsequently the hybrid is detected immunochemically with a horseradish peroxidase (HRP)-labeled antibody and tyramide signal amplification (catalyzed reporter deposition, CARD). After mRNA FISH, microorganisms can be identified by rRNA… CONTINUE READING

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