Simultaneous five cell-lineage flow cytometric analysis system for detection of leucocyte antibodies.

@article{Matsuyama2006SimultaneousFC,
  title={Simultaneous five cell-lineage flow cytometric analysis system for detection of leucocyte antibodies.},
  author={Nobuki Matsuyama and Yoshitaka Kojima and Fumiya Hirayama and Kazuta Yasui and Atsuko Taniue and Yasuo Fukumori and Keiji Yoshimura and Nobuko Tabata and Nobuo Sakata and Yoshihiko Tani and Hirotoshi Shibata},
  journal={Transfusion medicine},
  year={2006},
  volume={16 2},
  pages={111-8}
}
Although flow cytometric (FCM) analysis is one of the most widely used approaches to screen the presence of leucocyte antibodies, it has several drawbacks. First, neutrophils and, especially, monocytes exhibit high background reactivity. Second, to determine antibody specificity, it is often necessary to examine not only neutrophils and monocytes but also other lineage cells including T cells, B cells and platelets. Therefore, we attempted to establish an FCM analysis system in which four… CONTINUE READING