Simultaneous expression of recombinant baculovirus-encoded chicken anaemia virus (CAV) proteins VP1 and VP2 is required for formation of the CAV-specific neutralizing epitope.

@article{Noteborn1998SimultaneousEO,
  title={Simultaneous expression of recombinant baculovirus-encoded chicken anaemia virus (CAV) proteins VP1 and VP2 is required for formation of the CAV-specific neutralizing epitope.},
  author={Mathieu H. M. Noteborn and C A Verschueren and Grete Koch and Alex J van der Eb},
  journal={The Journal of general virology},
  year={1998},
  volume={79 ( Pt 12)},
  pages={3073-7}
}
Chicken anaemia virus (CAV) expresses three proteins, VP1, VP2 and VP3, but its capsid contains only the VP1 protein. In this paper, we report that for production of the neutralizing epitope, co-synthesis of (recombinant) VP1 and VP2 has to take place. We show via immunofluorescence that recombinant-baculovirus-infected Sf9 cells synthesizing VP1 (or VP2) alone react very poorly with CAV-specific neutralizing antibodies. In contrast, Sf9 cells co-infected with VP1- and VP2-recombinant… CONTINUE READING

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A manual of methods for baculovirus vectors and insect cell culture procedures

  • M. D. Summers, G. E. Smith
  • Texas Agricultural Experimental State Bulletin,
  • 1987
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