Simultaneous digital quantification and fluorescence-based size characterization of massively parallel sequencing libraries.

@article{Laurie2013SimultaneousDQ,
  title={Simultaneous digital quantification and fluorescence-based size characterization of massively parallel sequencing libraries.},
  author={Matthew T. Laurie and Jessica A. Bertout and Sean D. Taylor and Joshua N. Burton and Jay Shendure and Jason H. Bielas},
  journal={BioTechniques},
  year={2013},
  volume={55 2},
  pages={
          61-7
        }
}
Due to the high cost of failed runs and suboptimal data yields, quantification and determination of fragment size range are crucial steps in the library preparation process for massively parallel sequencing (or next-generation sequencing). Current library quality control methods commonly involve quantification using real-time quantitative PCR and size determination using gel or capillary electrophoresis. These methods are laborious and subject to a number of significant limitations that can… CONTINUE READING

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