Simultaneous determination of tramadol and its major active metabolite O-demethyltramadol by high-performance liquid chromatography with electrochemical detection.

@article{Valle1999SimultaneousDO,
  title={Simultaneous determination of tramadol and its major active metabolite O-demethyltramadol by high-performance liquid chromatography with electrochemical detection.},
  author={M. Del Valle and Jos{\'e} Manuel Cano Pav{\'o}n and Rosario Calvo and Miguel Angel Campanero and I{\~n}aki F. Troc{\'o}niz},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1999},
  volume={724 1},
  pages={83-9}
}
A novel, highly sensitive method was developed for simultaneous determination of tramadol and its main active metabolite O-demethyltramadol (ODMT) in rat plasma. The method involves a single-step extraction procedure and a specific determination by high-performance liquid chromatography with electrochemical detection, using an ethoxy analogue of tramadol (L… CONTINUE READING