Simultaneous determination of tiopronin and its metabolites in rat blood by LC-ESI-MS-MS using methyl acrylate for stabilization of thiol group.

@article{Matsuura2000SimultaneousDO,
  title={Simultaneous determination of tiopronin and its metabolites in rat blood by LC-ESI-MS-MS using methyl acrylate for stabilization of thiol group.},
  author={Keiko Matsuura and Keita Murai and Yasufumi Fukano and Hideo Takashina},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2000},
  volume={22 1},
  pages={101-9}
}
A sensitive and reproducible HPLC-electrospray tandem mass spectrometric method has been developed for the analysis of tiopronin (TP) and its metabolites, 2-mercaptopropionic acid (2-mpa) and S-methylated TP (SA13), in rat blood using methyl acrylate (MA) for the stabilization of a thiol group. The thiol groups of TP and 2-mpa in rat blood were immediately derivatized by the addition of MA-acetonitrile solution in 0.1 M Tris HCl (pH 9.1). The purification of the derivatives was accomplished by… CONTINUE READING
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