Simultaneous determination of pioglitazone and its two active metabolites in human plasma by LC-MS/MS.

@article{Lin2003SimultaneousDO,
  title={Simultaneous determination of pioglitazone and its two active metabolites in human plasma by LC-MS/MS.},
  author={Zhongping John Lin and Weihua Ji and Daksha Desai-Krieger and Linyee Shum},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2003},
  volume={33 1},
  pages={101-8}
}
A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of pioglitazone (PIO) and its two metabolites: M-III (keto-derivative) and M-IV (hydroxy-derivative) in human plasma. Human plasma samples of 0.2 ml were extracted by a single step liquid-liquid extraction procedure and analyzed using a high performance liquid chromatography (HPLC) electrospray tandem mass spectrometer system. The compounds were eluted isocratically… CONTINUE READING