Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection.

@article{Huang2007SimultaneousDO,
  title={Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection.},
  author={Hao Huang and Mingjin Liang and Xinmin Zhang and Chuan Zhang and Ziyin Shen and Weidong Zhang},
  journal={Journal of separation science},
  year={2007},
  volume={30 18},
  pages={3207-13}
}
A simple high performance liquid chromatographic method has been developed for the determination of nine flavonoids in Herba Epimedii, including quercetin-3-O-glucoside, epimedin A, hexandraside A, epimedin B, epimedin C, icariin, icariside I, icariside II, and icaritin. The chromatographic separation was performed on a Diamonsil C(18) column (5 microm, 250x4.6 mm) with gradient elution by acetonitrile-0.5% acetic buffer (adjusted to pH 5.00 with triethylamine). Methodological validation gave… CONTINUE READING