Simultaneous determination of malachite green and its metabolite leucomalachite green in eel plasma using post-column oxidation.

@article{Hajee1995SimultaneousDO,
  title={Simultaneous determination of malachite green and its metabolite leucomalachite green in eel plasma using post-column oxidation.},
  author={C. A. J. Hajee and Nel Haagsma},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1995},
  volume={669 2},
  pages={219-27}
}
A rapid HPLC method with solid-phase extraction (SPE) clean-up for malachite green (MG) and leucomalachite green (LMG) in eel plasma was developed. MG and LMG were extracted with a buffered methanolic solution. The extract was subjected to aromatic sulphonic acid SPE. MG and LMG were eluted from the SPE column with methanol after a treatment with ammonia gas. The reconstituted eluate was analyzed on a Chromspher B column with acetonitrile-ion-pair buffer (pH 4.0) (6:4, v/v) as the mobile phase… CONTINUE READING