Simultaneous determination of hydroxyeicosanoid (HETE) binding to cells and its cellular metabolism.


In order to study the mechanism(s) through which certain biologically active lipids, such as the hydroxyeicosanoids (HETEs), exert their effects, it is necessary to distinguish between binding of these lipids to cells and their cellular metabolism. A novel and simple method is described for the simultaneous determination of [3H]15-hydroxyeicosanoid (15-HETE… (More)