Simultaneous determination of hair cortisol, cortisone and DHEAS with liquid chromatography-electrospray ionization-tandem mass spectrometry in negative mode.

@article{Chen2013SimultaneousDO,
  title={Simultaneous determination of hair cortisol, cortisone and DHEAS with liquid chromatography-electrospray ionization-tandem mass spectrometry in negative mode.},
  author={Zheng Chen and Jifeng Li and Jing Zhang and Xue Min Xing and Wei Gao and Zuhong Lu and Huihua Deng},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2013},
  volume={929},
  pages={187-94}
}
The present study aimed to develop a novel method for simultaneous assay of cortisol, cortisone and dehydroepiandrosterone sulfate (DHEAS) in human hair. The method was based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-MS/MS) in negative mode. Analytes were extracted from 20-mg hair incubated in 1ml of methanol for 5 days. 100μl non-SPE supernatant of the incubation solution was utilized in LC-MS/MS analysis. The liquid chromatography separation was performed… CONTINUE READING