Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells.

@article{Tong2005SimultaneousDO,
  title={Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells.},
  author={Huaxiang Tong and Sarah A. Holstein and Raymond J. Hohl},
  journal={Analytical biochemistry},
  year={2005},
  volume={336 1},
  pages={51-9}
}
A sensitive, nonradioactive analytical method has been developed to simultaneously determine the concentrations of farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) in cultured cells. Following extraction, enzyme assays involving recombinant farnesyl protein transferase or geranylgeranyl protein transferase I are performed to conjugate FPP or GGPP to dansylated peptides. The reaction products are then separated and quantified by high-performance liquid chromatography coupled… CONTINUE READING