Simultaneous determination of carbohydrates and products of carbohydrate metabolism in fermentation mixtures by HPLC.

@article{Ross1987SimultaneousDO,
  title={Simultaneous determination of carbohydrates and products of carbohydrate metabolism in fermentation mixtures by HPLC.},
  author={L F Ross and D C Chapital},
  journal={Journal of chromatographic science},
  year={1987},
  volume={25 3},
  pages={112-7}
}
An improved procedure for separating and quantitating carbohydrates, alcohols, and organic acids in fermentation mixtures metabolized by intestinal microflora is described. The high-pressure liquid chromatographic method is efficient, reproducible, and sensitive. A column packed with cation-exchange resin in the hydrogen form, eluted isocratically with 0.028 M H2SO4 at 40 degrees C separates the compounds of interest. The eluate is monitored with ultraviolet and refractive index detectors in… CONTINUE READING