Simultaneous determination of OSI-774 and its major metabolite OSI-420 in human plasma by using HPLC with UV detection.

@article{Zhang2005SimultaneousDO,
  title={Simultaneous determination of OSI-774 and its major metabolite OSI-420 in human plasma by using HPLC with UV detection.},
  author={Wenjiang Zhang and Lillian L. Siu and Malcolm J. Moore and Eric Chen},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2005},
  volume={814 1},
  pages={143-7}
}
A new method was developed and validated for quantitating OSI-774 and its metabolite OSI-420 in human plasma. Sample preparation involved initial extraction with methyl t-butyl ether followed by back extraction with HCl and re-extraction with methyl t-butyl ether. This extraction process resulted in significant improvement in the specificity, reproducibility and sensitivity. The analytes were separated on a Water Symmetry C18 analytical column and the mobile phase consisted of acetonitrile-0.05… CONTINUE READING

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