Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC.

@article{Dervieux1998SimultaneousDO,
  title={Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC.},
  author={T Dervieux and Roselyne Boulieu},
  journal={Clinical chemistry},
  year={1998},
  volume={44 3},
  pages={551-5}
}
6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the simultaneous determination of 6-TGNs and Me6-MPNs in erythrocytes. A simple and rapid treatment procedure based on deproteinization by perchloric acid with dithiothreitol is… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 12 references

High performance liquid chromatographic determination of thiopurine metabolites of azathioprine in biological fluids

R Boulieu, A. Lenoir
J Chromatogr • 1993
View 3 Excerpts
Highly Influenced

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

CW Keuzenkamp-Jansen, RA De Abreu, JPM Bokkering, JMF. Trijbels
J Chromatogr B Biomed Appl 1995:72:53–61 • 1995

The importance of methylthio-IMP for methylmercaptopurine ribonucleoside (Me-MPr) cytotoxicity in Molt F4 human malignant T lymphoblasts

M Vogt, E Stet, +3 authors J. Trijbels
Biochim Biophys Acta 1993:1181:189–94 • 1993
View 1 Excerpt

The clinical pharmacology of 6-mercaptopurine

European Journal of Clinical Pharmacology • 1992
View 1 Excerpt

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