Simultaneous determination of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in exhaled breath condensate by hydrophilic interaction liquid chromatography/electrospray ionization tandem mass spectrometry.

@article{Conventz2007SimultaneousDO,
  title={Simultaneous determination of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in exhaled breath condensate by hydrophilic interaction liquid chromatography/electrospray ionization tandem mass spectrometry.},
  author={Angela Conventz and Anita K Musiol and C Brodowsky and Alice M{\"u}ller-Lux and Petra Dewes and Thomas Kraus and T Schettgen},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2007},
  volume={860 1},
  pages={78-85}
}
The analysis of biomarkers from exhaled breath condensate (EBC) is a non-invasive but challenging method for the detection of pulmonary diseases. The amino acids L-proline (Pro) and l-tyrosine (Tyr) are precursors for two important metabolites, trans-L-4-hydroxyproline (trans-L-4-hydroxypyrrolidin-2-carboxylic acid, t-Hyp) and nitrotyrosine (NT). Whereas t-Hyp is supposed to be a biomarker for lung fibrosis, NT is a promising biomarker for inflammation in airway diseases. Analysis of EBC… CONTINUE READING