Simultaneous detection of three fish rhabdoviruses using multiplex real-time quantitative RT-PCR assay.

@article{Liu2008SimultaneousDO,
  title={Simultaneous detection of three fish rhabdoviruses using multiplex real-time quantitative RT-PCR assay.},
  author={Zongxiao Liu and Yong Teng and Hong Liu and Yulin Jiang and Xiayang Xie and Huifang Li and Jiangqiang Lv and Longying Gao and Junqiang He and Xiujie Shi and Feiyan Tian and Jingshun Yang and Congxin Xie},
  journal={Journal of virological methods},
  year={2008},
  volume={149 1},
  pages={103-9}
}
Spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) are three important fish rhabdoviruses, causing serious Office International des Epizooties (OIE) classified diseases in wild and farmed fish. Here, a new multiplex real-time quantitative RT-PCR (mqRT-PCR) assay was developed for simultaneous detection, identification and quantification of these three rhabdoviruses. The sets of primers and probes were targeted to… CONTINUE READING