Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation: polymerase chain reaction and immunocytochemical studies at the single cell level.

@article{Liu1994SimultaneousDO,
  title={Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation: polymerase chain reaction and immunocytochemical studies at the single cell level.},
  author={Fu-Hwa Liu and Luc Malaval and Ashish Gupta and Jane E. Aubin},
  journal={Developmental biology},
  year={1994},
  volume={166 1},
  pages={220-34}
}
Messenger RNA expression analyzed by in situ hybridization and Northern analysis and protein expression analyzed biochemically or immunocytochemically have been used to study the developmental expression of various osteoblast (OB)-associated molecules. These approaches have shown that over a time course of OB differentiation in vivo and in vitro, the expression of macromolecules associated with OB cells changes. However, ambiguities in data from different approaches and in populations… CONTINUE READING

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