Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application.

Abstract

We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clinical stool specimens.

DOI: 10.1128/JCM.00245-14

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Cite this paper

@article{Jiang2014SimultaneousDO, title={Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application.}, author={Yanhong Jiang and Lin Fang and Xiaolu Shi and Hailong Zhang and Yinghui Li and Yiman Lin and Yaqun Qiu and Qingliang Chen and Hui Li and Li Zhou and Qinghua Hu}, journal={Journal of clinical microbiology}, year={2014}, volume={52 4}, pages={1266-8} }